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RESEARCH ARTICLE

Impact of relative humidity on SARS-CoV-2 RNA extraction using Nextractor automated extraction system

Kumar Jain Rajeev, Sharma Archa, Lalwani Jaya, Chaurasia Deepti, Perumal Nagaraj
JBM 2024, 11(2), e99010012 https://doi.org/10.14440/jbm.2024.0001
16 Download 176 Views
BENCHMARK

Benchmarking assembly free nanopore read mappers to classify complex millipede gut microbiota via Oxford Nanopore Sequencing Technology

J. Geli-Cruz Orlando, J. Santos-Flores Carlos, J. Cafaro Matias, Ropelewski Alex, R. Van Dam Alex
12 Download 134 Views
PROTOCOLS

Prevention, Diagnosis and Eradication of Mycoplasma Contamination in Cell Culture

Huang Xuefeng , Yu Minghang , Wang Bingbing , Zhang Yanlong , ... Xi Wang
24 Download 160 Views
PROTOCOLS

A strategy to recover a poor-quality ligase product

Prete Sonia Del, Gogliettino Marta, Palmieri Gianna, Cocca Ennio
6 Download 467 Views
PROTOCOLS

Proximity Protein Labeling In Dictyostelium With Engineered Ascorbic Acid Peroxidase 2

A. Takashima Jamie, A. Woroniecka Helena, G. Charest Pascale
1 Download 114 Views
ARTICLE

Utilizing a human TLR selective ligand in a humanized immune system mouse model to investigate human TLR4 signaling

Twomey Rachel , Graham Sean , S. Spina Joseph , Wu Xiaoming , ... William Housley
13 Download 471 Views
PROTOCOLS

Multiplexed tape-stabilized cryohistology of mineralized large animal specimens

M. Zlotnick Hannah, Jiang Xi, L. Mauck Robert, A. Dyment Nathaniel
103 Views
PROTOCOLS

SpheroidAnalyseR—an online platform for analyzing data from 3D spheroids or organoids grown in 96-well plates

Barrow Rhiannon , N. Wilkinson Joseph , He Yichen , Callaghan Martin , ... Lucy F. Stead
2 Download 172 Views
ARTICLE

Measurement of skeletal muscle glucose uptake in mice in response to acute treadmill running

Møller Lisbeth Liliendal Valbjørn, Raun Steffen Henning, Fritzen Andreas Mæchel, Sylow Lykke
1 Download 180 Views
PROTOCOLS

Site-specific nanobody-oligonucleotide conjugation for super-resolution imaging

Teodori Laura , Omer Marjan , Märcher Anders , K. Skaanning Mads , ... Jørgen Kjems
163 Views
PROTOCOLS

Development of a reproducible porcine model of infected burn wounds

Said Sayf Al-deen , Jatana Samreen , K. Ponti András , E. Johnson Erin , ... Christine McDonald
1 Download 160 Views
PROTOCOLS

Novel adaptation of a running suture technique in a mouse model of corneal transplantation

Nakagawa Hayate , Blanco Tomas , Kahale Francesca , Bir Singh Rohan , ... Reza Dana
JBM 2021, 8(4), 1 https://doi.org/0
0 Views
ARTICLE

Tissue-specific DamID protocol using nanopore sequencing

Gómez-Saldivar Georgina, A. Glauser Dominique, Meister Peter
1 Download 92 Views
PROTOCOLS

An efficient method to generate kidney organoids at the air-liquid interface

Kumar Gupta Ashwani, Z. Ivancic David, A. Naved Bilal, A. Wertheim Jason, Oxburgh Leif
151 Views
PROTOCOLS

In vitro fermentation test bed for evaluation of engineered probiotics in polymicrobial communities

Arcidiacono Steven , M. Ehrenworth Breedon Amy , S. Goodson Michael , A. Doherty Laurel , ... Jason W. Soares
157 Views
ARTICLE

High throughput nanopore sequencing of SARS-CoV-2 viral genomes from patient samples

A. Pater Adrian , S. Bosmeny Michael , A. White Adam , J. Sylvain Rourke , ... Keith T. Gagnon
JBM 2021, 8(19), 1 https://doi.org/0
0 Views
BENCHMARK

Optimization of small-scale sample preparation for high-throughput OpenArray analysis

A. Abraham Neeta, C. Campbell Anne, D. Hirst Warren, L. Nezich Catherine
161 Views
PROTOCOLS

Fabricating spatially functionalized 3D-printed scaffolds for osteochondral tissue engineering

Camacho Paula, Fainor Matthew, B. Seims Kelly, W. Tolbert John, W. Chow Lesley
1 Download 92 Views
PROTOCOLS

Using a variant of the optomotor response as a visual defect detection assay in zebrafish

K. LeFauve Matthew, J. Rowe Cassie, Crowley-Perry Mikayla, L. Wiegand Jenna, G. Shapiro Arthur
88 Views
ARTICLE

A simplified design for the C. elegans lifespan machine

Abbott Mark , A. Banse Stephen , Melentijevic Ilija , M. Jarrett Cody , ... Monica Driscoll
1 Download 159 Views
PROTOCOLS

A simple and robust cell-based assay for the discovery of novel cytokinesis inhibitors

Radnai Laszlo , F. Stremel Rebecca , Vaissiere Thomas , Lin Li , ... Courtney A. Miller
166 Views
ARTICLE

An in vitro model of hepatic steatosis using lipid loaded induced pluripotent stem cell derived hepatocyte like cells

Deepak Hiraganahalli Bhaskar , Shreekrishna Nellikalaya , Sameermahmood Zaheerbasha , Anand Niranjan Naranapur , ... Saravanakumar Dhakshinamoorthy
91 Views
ARTICLE

A 2D and 3D melanogenesis model with human primary cells induced by tyrosine

S. Branquinho Maryana , B. Silva Maysa , C. Silva Jacqueline , C. Sales Maria , ... Ana Campa
151 Views
ARTICLE

Real time visualization of cancer cell death, survival and proliferation using fluorochrome-transfected cells in an IncuCyte® imaging system

M. Lanigan Thomas , M. Rasmussen Stephanie , P. Weber Daniel , S. Athukorala Kalana , ... Jeffrey H. Ruth
2 Download 93 Views
ARTICLE

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells

Manuszak Claire, Brainard Martha, Thrash Emily, Hodi F. Stephen, Severgnini Mariano
159 Views
PROTOCOLS

A simple method for short-term maintenance of neonatal mice without foster mothers

Kreikemeier-Bower Craig, Polepole Pascal, Pinkerton Katherine, Zhang Luwen
93 Views
ARTICLE

An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins

D. Roberts Michael , C. Young Kaelin , D. Fox Carlton , G. Vann Christopher , ... Andreas N. Kavazis
92 Views
BENCHMARK

Comparative analysis of diagnostic platforms for measurement of differentially methylated insulin DNA

J. Farr Ryan , K. M. Wong Wilson , Maynard Cody-lee , A. Tersey Sarah , ... Mugdha V. Joglekar
160 Views
ARTICLE

Detection of clinically relevant immune checkpoint markers by multicolor flow cytometry

A. Cunningham Rachel, Holland Martha, McWilliams Emily, Hodi Frank Stephen, Severgnini Mariano
149 Views
PROTOCOLS

A step-by-step beginner’s protocol for whole genome sequencing of human bacterial pathogens

S. Gautam Sanjay, KC Rajendra, WC Leong Kelvin, Aogáin Micheál Mac, F. O’Toole Ronan
1 Download 157 Views
ARTICLE

Real time mitochondrial dimension measurements

M. Leichner Joseph, Konyukhov Evgene, Kamoun David, Yaniv Yael
99 Views
ARTICLE

A minimally-invasive serial cerebrospinal fluid sampling model in conscious Göttingen minipigs

Bergadano Alessandra , Amen Eva Maria , Jacobsen Björn , Belli Sara , ... Claudia Senn
93 Views
ARTICLE

An automated quantitative image analysis pipeline of in vivo oxidative stress and macrophage kinetics

D. Paredes Andre , Benavidez David , Cheng Jun , Mangos Steve , ... Amelia Bartholomew
91 Views
ARTICLE

A model for the detection of pancreatic ductal adenocarcinoma circulating tumor cells

S. Alexander Matthew , R. O’Leary Brianne , Moose Devon , Du Juan , ... Joseph J. Cullen
83 Views
PROTOCOLS

Large-scale production of lentiviral vectors using multilayer cell factories

Rout-Pitt Nathan, McCarron Alexandra, McIntyre Chantelle, Parsons David, Donnelley Martin
82 Views
ARTICLE

Development and validation of an allele-specific PCR assay for genotyping a promoter and exonic single nucleotide polymorphisms of MGMT gene

Tyagi Anuj Kumar, Khoshbeen Mary Boudal, Curtis Patricia Huezo-Diaz, S. Uppugunduri Chakradhara Rao, Ansari Marc
81 Views
PROTOCOLS

Streamlined preparation of genomic DNA in agarose plugs for pulsed-field gel electrophoresis

Hicks Linda , M. van der Graa Charlotte , Childress Jacob , Cook Emily , ... Eugene Kroll
86 Views
ARTICLE

Insulin receptor based lymphocyte trafficking in the progression of type 1 diabetes

P. Morran Michael , G. Al-Dieri Ali , L. Nestor‐Kalinoski Andrea , K. Jordan Richard , ... Marcia F. McInerney
81 Views
REVIEW

Elucidating endotoxin-biomolecule interactions with FRET: extending the frontiers of their supramolecular complexation

M. Obeng Eugene, C. Dullah Elvina, Razak Nur Syahadatain Abdul, K. Danquah Michael, Budiman Cahyo
1 Download 164 Views
PROTOCOLS

RATA: A method for high-throughput identification of RNA bound transcription factors

Schmidt Karyn, Buquicchio Frank, S. Carroll Johanna, J. Distel Robert, D. Novina Carl
87 Views
ARTICLE

A method for evaluating cocaine-induced social preference in rats

M. Dingess Paige, J. Deters Morgan, A. Darling Rebecca, A. Yarborough Erin, E. Brown Travis
90 Views
BENCHMARK

Performance of pyrosequencing versus MALDI-TOF MS in bacteria identification in chronic lung disease

Navrátilová Lucie , Procházková Petra , Bardoň Jan , Novotný Radko , ... Vladislav Raclavský
84 Views
PROTOCOLS

Analysis of the translatome in solid tumors using polyribosome profiling/RNA-Seq

Adjibade Pauline , St-Sauveur Valérie Grenier , Droit Arnaud , W. Khandjian Edouard , ... Rachid Mazrou
85 Views
PROTOCOLS

Protocol for evaluation of neurotrophic strategies in Parkinson

V. Hegarty Shane, M. Sullivan Aideen, W. O’Keeffe Gerard
81 Views
PROTOCOLS

Direct lentivirus injection for fast and efficient gene transfer into brown and beige adipose tissue

Balkow Aileen , S. Hoffmann Linda , Klepac Katarina , Glöde Anja , ... Alexander Pfeifer
80 Views
PROTOCOLS

Establishment of a Drosophila AD model

Wang Xingjun , Zhao Yu , Hu Yujia , Ren Pu , ... Lei Xue
1 Download 146 Views
PROTOCOLS

Assessing bioenergetic functions from isolated mitochondria in Drosophila melanogaster

C. Aw Wen, Bajracharya Rijan, G. Towarnicki Samuel, O. Ballard J. William
89 Views
PROTOCOLS

Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects

S. Rozas-Dennis Gabriela, A. Marti Gerardo, González Nadia Lorena, Guhl Felipe, M. A. Guérin Diego
76 Views
ARTICLE

Concentrating white spot syndrome virus by alum for field detection using a monoclonal antibody based flow-through assay

Rani Amrita , R. Poojary Sathish , B. Thammegouda Naveen Kumar , P. Ballyaya Abhiman , ... Santhosh K. Shivakumaraswamy
75 Views
ARTICLE

Development of a method for DNA extraction from oil palm leaves and the effects of pH and ionic strength on nucleic acid quantification

Ihase Leonard Osayande , Horn Renate , Anoliefo Geoffrey Obinna , Ralph Eke Chukwuemeka , ... Omorefe Asemota
78 Views
APPLICATION NOTE

CellTrace™ Far Red & CellTracker™ Deep Red

Zhou Wenjun , Kang Hee Chol , O’Grad Mike , M. Chamber Kevin , ... Kyle R. Gee
1 Download 81 Views
ARTICLE

Versatile microsphere attachment of GFP-labeled motors and other tagged proteins with preserved functionality

Bugiel Michael , Fantana Horatiu , Bormuth Volker , Trushko Anastasiya , ... Anita Jannasch
149 Views
REVIEW

Enzymatic processes in alternative reaction media: a mini review

Ghaffari-Moghaddam Mansour, Eslahi Hassan, A. Aydin Yasar, Saloglu Didem
2 Download 152 Views
PROTOCOLS

Ocular surgical models for immune and angiogenic responses

Inomata Takenori, Mashaghi Alireza, Di Zazzo Antonio, Dana Reza
82 Views
ARTICLE

Open source and DIY hardware for DNA nanotechnology labs

R. Damase Tulsi, Stephens Daniel, Spencer Adam, B. Allen Peter
1 Download 86 Views
ARTICLE

Deciphering hepatocellular responses to metabolic and oncogenic stress

L. Marcelo Kathrina , Lin Fumin , Rajapakshe Kimal , Dean Adam , ... Brian York
82 Views
ARTICLE

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

Martínez-Márquez Ascensión , Morante-Carriel Jaime , Ramírez-Estrada Karla , M. Cusidó Rosa , ... Roque Bru-Martínez
77 Views
PROTOCOLS

Inducing gene expression by targeting promoter sequences using small activating RNAs

Wang Ji , F. Place Robert , Portnoy Victoria , Huang Vera , ... Long-Cheng Li
84 Views
ARTICLE

Assessing phytase activity–methods, definitions and pitfalls

Qvirist Linnea, Carlsson Nils-Gunnar, Andlid Thomas
1 Download 91 Views
PROTOCOLS

Use of retroviral-mediated gene transfer to deliver and test function of chimeric antigen receptors in human T-cells

C. Parente-Pereira Ana, Wilkie Scott, J.C. van der Stegen Sjoukje, M. Davies David, Maher John
80 Views
PROTOCOLS

Advanced methods of microscope control using μManager software

D. Edelstein Arthur , A. Tsuchida Mark , Amodaj Nenad , Pinkard Henry , ... Nico Stuurman
85 Views
PROTOCOLS

Isolating astrocytes and neurons sequentially from postnatal murine brains with a magnetic cell separation technique

Feldmann Maria, Pathipati Praneeti, Sheldon R. Ann, Jiang Xiangning, M. Ferriero Donna
1 Download 91 Views
ARTICLE

A noninvasive assay for monitoring renal allograft status

Romanov Victor, Whyard Terry C., Waltze Wayne C., S. Darras Frank
1 Download 82 Views
REVIEW

Diagnosis of Alzheimer's disease: Towards accuracy and accessibility

Mu Yan , Chang Ke-Xin , Chen Yu-Feng , Yan Ke , ... Qian Hua
30 Download 232 Views
ARTICLE

Development of an In Situ Hybridization Method for Detection of Akabane Virus

Toplu Nihat, Oğuzoğlu uba Çiğdem, Akkoç Ayşe Nur
18 Download 181 Views
ARTICLE

Validation of 12 Rapid Antigen Tests for the Detection of SARS-CoV-2

Yu Minghang , Xiong Yang , Liang Pu , Chen Danying , ... Xi Wang
31 Download 394 Views
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Journal of Biological Methods, Electronic ISSN: 2326-9901 Print ISSN: TAB, Published by POL Scientific