Benchmarking assembly free nanopore read mappers to classify complex millipede gut microbiota via Oxford Nanopore Sequencing Technology
Prevention, Diagnosis and Eradication of Mycoplasma Contamination in Cell Culture
An experimental workflow for identifying RNA m6A alterations in cellular senescence by methylated RNA immunoprecipitation sequencing
A strategy to recover a poor-quality ligase product
Proximity Protein Labeling In Dictyostelium With Engineered Ascorbic Acid Peroxidase 2
Optimizing factors for large-scale production of Arbuscular Mycorrhizal Fungi consortia using root organ cultures
An experimental workflow for enrichment of low abundant proteins from human serum for the discovery of serum biomarkers
Utilizing a human TLR selective ligand in a humanized immune system mouse model to investigate human TLR4 signaling
Multiplexed tape-stabilized cryohistology of mineralized large animal specimens
SpheroidAnalyseR—an online platform for analyzing data from 3D spheroids or organoids grown in 96-well plates
Measurement of skeletal muscle glucose uptake in mice in response to acute treadmill running
Estimating somatic mutation rates by bottlenecked duplex sequencing in non-model organisms: Daphnia magna as a case study
An experimental method for evoking and characterizing dynamic color patterning of cuttlefish during prey capture
Methodology for measuring oxidative capacity of isolated peroxisomes in the Seahorse assay
Site-specific nanobody-oligonucleotide conjugation for super-resolution imaging
Development of a reproducible porcine model of infected burn wounds
Novel adaptation of a running suture technique in a mouse model of corneal transplantation
Comparison of CRISPR and adenovirus-mediated Myd88 knockdown in RAW 264.7 cells and responses to lipopolysaccharide stimulation
Tissue-specific DamID protocol using nanopore sequencing
Getting two birds with one stone: Combining immunohistochemistry and Azan staining in animal morphology
A method for the efficient iron-labeling of patient-derived xenograft cells and cellular imaging validation
An efficient method to generate kidney organoids at the air-liquid interface
Assessment of galactose-1-phosphate uridyltransferase activity in cells and tissues
In vitro fermentation test bed for evaluation of engineered probiotics in polymicrobial communities
Special issue editorial: Methods to facilitate SARS-CoV-2 and COVID-19 research
Virtual screening on the web for drug repurposing: a primer
High throughput nanopore sequencing of SARS-CoV-2 viral genomes from patient samples
Monobiotinylated Proteins Tethered to Microspheres for Detection of Antigen-Specific Serum Antibodies
Optimization of small-scale sample preparation for high-throughput OpenArray analysis
Fabricating spatially functionalized 3D-printed scaffolds for osteochondral tissue engineering
Using a variant of the optomotor response as a visual defect detection assay in zebrafish
High-yield purification of exceptional-quality, single-molecule DNA substrates
Visualization of subdiffusive sites in a live single cell
An ex vivo skin model to probe modulation of local cutaneous arachidonic acid inflammation pathway
Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids
A streamlined CRISPR/Cas9 approach for fast genome editing in Toxoplasma gondii and Besnoitia besnoiti
An adapted novel flow cytometry methodology to delineate types of cell death in airway epithelial cells
A simplified design for the C. elegans lifespan machine
A simple and robust cell-based assay for the discovery of novel cytokinesis inhibitors
An in vitro model of hepatic steatosis using lipid loaded induced pluripotent stem cell derived hepatocyte like cells
A 2D and 3D melanogenesis model with human primary cells induced by tyrosine
Expansion and cellular characterization of primary human adherent cells in the Quantum® Cell Expansion System, a hollow-fiber bioreactor system
Real time visualization of cancer cell death, survival and proliferation using fluorochrome-transfected cells in an IncuCyte® imaging system
A simple method for long-term vital-staining of ciliated epidermal cells in aquatic larvae
Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells
Generation of bone marrow chimeras using X-ray irradiation: comparison to cesium irradiation and use in immunotherapy
Comparing NGS and NanoString platforms in peripheral blood mononuclear cell transcriptome profiling for advanced heart failure biomarker development
A simple method for short-term maintenance of neonatal mice without foster mothers
Highly efficient induced pluripotent stem cell reprogramming of cryopreserved lymphoblastoid cell lines
A system for the high-throughput analysis of acute thermal avoidance and adaptation in C. elegans
Optimized methodology for product recovery following emulsion PCR: applications for amplification of aptamer libraries and other complex templates
An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins
Corrigendum: A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
A standardized methodology for longitudinal assessment of corneal endothelial morphometry in eye banked corneas
A multivariate statistical analysis of the effects of styrene maleic acid encapsulated RL71 in a xenograft model of triple negative breast cancer
FairSubset: A tool to choose representative subsets of data for use with replicates or groups of different sample sizes
Embryo microinjection of the lecithotrophic sea urchin Heliocidaris erythrogramma
The answer depends on the question: Optimal conditions for western blot characterization of muscle collagen type 1 depends on desired isoform
In vivo measurement of enhanced agouti-related peptide release in the paraventricular nucleus of the hypothalamus through Gs activation of agouti-related peptide neurons
Comparative analysis of diagnostic platforms for measurement of differentially methylated insulin DNA
Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastoma
Primary cell-based phenotypic assays to pharmacologically and genetically study fibrotic diseases in vitro
Detection of clinically relevant immune checkpoint markers by multicolor flow cytometry
A step-by-step beginner’s protocol for whole genome sequencing of human bacterial pathogens
Real time mitochondrial dimension measurements
Inflammatory monocyte response due to altered wall shear stress in an isolated femoral artery model
Small RNA-seq: The RNA 5’-end adapter ligation problem and how to circumvent it
A minimally-invasive serial cerebrospinal fluid sampling model in conscious Göttingen minipigs
Oxidation of cysteine-rich proteins during gel electrophoresis
Method for selective quantification of immune and inflammatory cells in the cornea using flow cytometry
A rapid, high-throughput method for determining chronological lifespan in budding yeast
High resolution measurement of membrane receptor endocytosis
A highly-simplified and inexpensive MALDI-TOF mass spectrometry sample-preparation method with broad applicability to microorganisms, plants, and insects
An automated quantitative image analysis pipeline of in vivo oxidative stress and macrophage kinetics
Improved production of monoclonal antibodies against the LcrV antigen of Yersinia pestis using FACS-aided hybridoma selection
RNA isolation from Peyer's patch lymphocytes and mononuclear phagocytes to determine gene expression profiles using NanoString technology
Conditioned contextual fear memory to assess natural forgetting and cognitive enhancement in rats
Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing
A model for the detection of pancreatic ductal adenocarcinoma circulating tumor cells
Exosomes molecular diagnostics: Direct conversion of exosomes into the cDNA for gene amplification by two-step polymerase chain reaction
Development and implementation of a cell-based assay to discover agonists of the nuclear receptor REV-ERBα
Use of chick neural tube for optimizing the PSM and epithelial somites electroporation parameters: A detailed protocol
Patient-derived xenograft model for uterine leiomyoma by sub-renal capsule grafting
Large-scale production of lentiviral vectors using multilayer cell factories
Development and validation of an allele-specific PCR assay for genotyping a promoter and exonic single nucleotide polymorphisms of MGMT gene
A modified rat model of hindlimb ischemia for augmentation and functional measurement of arteriogenesis
Isolation, cultivation and immunostaining of single myofibers: An improved approach to study the behavior of satellite cells
Streamlined preparation of genomic DNA in agarose plugs for pulsed-field gel electrophoresis
Agrobacterium-mediated transformation of Camelina sativa for production of transgenic plants
Histone deacetylase inhibitor-based chromatin precipitation for identification of targeted genomic loci
Insulin receptor based lymphocyte trafficking in the progression of type 1 diabetes
Analysis of gene expression in filamentous cells of Candida albicans grown on agar plates
Easy and cost-effective stable positioning of suspension cells during live-cell imaging
A bi-functional IL-6-HaloTag® as a tool to measure the cell-surface expression of recombinant odorant receptors and to facilitate their activity quantification
IL-6–HaloTag® enables live-cell plasma membrane staining, flow cytometry, functional expression, and deorphaning of recombinant odorant receptors
Aspiration techniques for bronchoalveolar lavage in translational respiratory research: Paving the way to develop novel therapeutic moieties
Measurement of intracellular calcium of submandibular glands using a high throughput plate reader
Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
A multiplex PCR strategy to screen for known mutations in families with sudden cardiac death burden
A fluorescence assay for detecting amyloid-β using the cytomegalovirus enhancer/promoter
Small peptide substrates and high resolution peptide gels for the analysis of site-specific protein phosphorylation and dephosphorylation
A mass spectrometric method for quantification of tryptophan-derived uremic solutes in human serum
Elucidating endotoxin-biomolecule interactions with FRET: extending the frontiers of their supramolecular complexation
A cost-effective RNA extraction technique from animal cells and tissue using silica columns
Resolving biofilm topography by native scanning electron microscopy
Total proteome turbidity assay for tracking global protein aggregation in the natural cellular environment
β-mercaptoethanol assists efficient construction of sperm bacterial artificial chromosome library
RATA: A method for high-throughput identification of RNA bound transcription factors
Combining FRET and optical tweezers to study RhoGTPases spatio-temporal dynamics upon local stimulation
A guide to simple, direct, and quantitative in vitro binding assays
A fast and reproducible cell- and 96-well plate-based method for the evaluation of P2X7 receptor activation using YO-PRO-1 fluorescent dye
Using cluster edge counting to aggregate iterations of centroid-linkage clustering results and avoid large distance matrices
A method for evaluating cocaine-induced social preference in rats
CRISPR Explorer: A fast and intuitive tool for designing guide RNA for genome editing
Performance of pyrosequencing versus MALDI-TOF MS in bacteria identification in chronic lung disease
Analysis of the translatome in solid tumors using polyribosome profiling/RNA-Seq
A practical approach to remote ischemic preconditioning and ischemic preconditioning against myocardial ischemia/reperfusion injury
A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
Incorporation of β-actin loading control into zymography
Quantitative histological image analyses of reticulin fibers in a myelofibrotic mouse
Method for selective quantification of adipose-derived stromal/stem cells in tissue
Method to extract minimally damaged collagen fibrils from tendon
A rapid fluorometric assay for the S-malonyltransacylase FabD and other sulfhydryl utilizing enzymes
Serum proteins are extracted along with monolayer cells in plasticware and interfere with protein analysis
Assessing zygosity in progeny of transgenic plants: current methods and perspectives
Protocol for evaluation of neurotrophic strategies in Parkinson
A three-dimensional co-culture system to investigate macrophage-dependent tumor cell invasion
Direct lentivirus injection for fast and efficient gene transfer into brown and beige adipose tissue
Sample preparation for proteomic analysis using a GeLC-MS/MS strategy
High-contrast enzymatic immunohistochemistry of pigmented tissues
Establishment of a Drosophila AD model
Assessing bioenergetic functions from isolated mitochondria in Drosophila melanogaster
Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
Inexpensive, serotype-independent protocol for native and bioengineered recombinant adeno-associated virus purification
Concentrating white spot syndrome virus by alum for field detection using a monoclonal antibody based flow-through assay
A comparison of strategies for immortalizing mouse embryonic fibroblasts
Development of a method for DNA extraction from oil palm leaves and the effects of pH and ionic strength on nucleic acid quantification
ChIP and Chips: Introducing the WormPharm for correlative studies employing pharmacology and genome-wide analyses in C. elegans
Corrigendum: Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR)
CellTrace™ Far Red & CellTracker™ Deep Red
Removing nucleic acids from nucleoid-Associated proteins purified by affinity column
A surface plasmon resonance-based method for monitoring interactions between G protein-coupled receptors and interacting proteins
Application of linker technique to trap transiently interacting protein complexes for structural studies
Comprehensive analysis of signal transduction in three-dimensional ECM-based tumor cell cultures
Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR)
Co-culture of placental explants with isolated CD4 and CD8 T cells: a functional model to define the consequences of placental inflammation
Versatile microsphere attachment of GFP-labeled motors and other tagged proteins with preserved functionality
Enzymatic processes in alternative reaction media: a mini review
Reliable and inexpensive expression of large, tagged, exogenous proteins in murine bone marrow-derived macrophages using a second generation lentiviral system
Ocular surgical models for immune and angiogenic responses
Open source and DIY hardware for DNA nanotechnology labs
Deciphering hepatocellular responses to metabolic and oncogenic stress
Cell-based assays using calcein acetoxymethyl ester show variation in fluorescence with treatment conditions
Isolation and quantification of N-glycans from immunoglobulin G antibodies for quantitative glycosylation analysis
A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media
Extraction of RNA from tough tissues with high proteoglycan content by cryosection, second phase separation and high salt precipitation
A quantitative, surface plasmon resonance-based approach to evaluating DNA binding by the c-Myc oncoprotein and its disruption by small molecule inhibitors
Fluorescent foci quantitation for high-throughput analysis
Generation of axolotl hematopoietic chimeras
Inducing gene expression by targeting promoter sequences using small activating RNAs
Silk coating as a novel delivery system and reversible adhesive for stiffening and shaping flexible probes
A simple, effective and inexpensive method to highlight antigen-antibody reaction based on liquid-semisolid phase
Assessing phytase activity–methods, definitions and pitfalls
In vitro and in vivo model systems used in prostate cancer research
Use of retroviral-mediated gene transfer to deliver and test function of chimeric antigen receptors in human T-cells
Method for the quantitative measurement of collecting lymphatic vessel contraction in mice
A stepwise procedure to test contractility and susceptibility to injury for the rodent quadriceps muscle
Advanced methods of microscope control using μManager software
Isolating astrocytes and neurons sequentially from postnatal murine brains with a magnetic cell separation technique
Proof of concept for a novel functional screening system for plant sucrose effluxers
Automated high-throughput measurement of body movements and cardiac activity of Xenopus tropicalis tadpoles
A stepwise procedure for isolation of murine bone marrow and generation of dendritic cells
A noninvasive assay for monitoring renal allograft status
Anomalous Subdiffusive Measurements by Fluorescence Correlations Spectroscopy and Simulations of Translational Diffusive Behavior in Live Cells
Scientific methods – the foundation of science
Diagnosis of Alzheimer's disease: Towards accuracy and accessibility
Development of an In Situ Hybridization Method for Detection of Akabane Virus
Validation of 12 Rapid Antigen Tests for the Detection of SARS-CoV-2