POL Scientific / JBM / Volume 12 / Issue 2 / DOI: 10.14440/jbm.2024.0116
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RESEARCH ARTICLE

Correlation between antibacterial activities of two Artemisia spp. extracts and their plant characteristics

Abdullah Mashraqi1 ,  Mohamed A. Al Abboud1 ,  Khatib Sayeed Ismail1 ,  Yosra Modafer1 ,  Mukul Sharma2 ,  A. El-Shabasy1*
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1 Department of Biology, College of Science, Jazan University, Jazan 45142, Kingdom of Saudi Arabia
2 Environment and Nature Research, Jazan University, Jazan 45142, Kingdom of Saudi Arabia
JBM 2025 , 12(2), e99010057; https://doi.org/10.14440/jbm.2024.0116
Submitted: 13 November 2024 | Revised: 6 February 2025 | Accepted: 14 February 2025 | Published: 19 June 2025
© 2025 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Background: The antimicrobial activity of Artemisia absinthium L. and Artemisia herba-alba Asso. against various pathogens is differentiated by using different organic solvents and aqueous solution and in terms of pollen grain traits. The similarities and dissimilarities were analyzed by simple linear regressions and in terms of Pearson correlation coefficients. Objective: The present study evaluated the potential antibacterial activity of A. absinthium L. and A. herba-alba Asso. extracts by using various organic and aqueous solvents. The tested bacteria included pathogenic strains: Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus. Methods: Different affinities were observed for the studied organic solvents in addition to aqueous ones. A comparative analysis was conducted, focusing morphological, anatomical, and palynological characteristics. The similarity parameter was obtained. The minimum inhibitory concentration (MIC) values for both plant extracts were analyzed using the analysis of variance, while Pearson correlation coefficients were calculated for plant traits. Results: Butanol emerged as the predominant organic solvent extract for both species whereas chloroform and diethyl ether exhibited a broad antibacterial spectrum for A. absinthium L. and A. herba alba Asso. MIC and minimum bactericidal concentration values were confirmed by using butanol and diethyl ether extracts of A. absinthium L. and butanol and chloroform extracts of A. herba alba Asso. against the tested pathogenic bacteria. The results highlight the potential of these extracts as alternative natural antibacterial agents. Conclusion: This study demonstrated that using successive organic plant extractions can help identify the most effective extract that can serve as a source of alternative medicine due to its various active natural components.

Keywords
Listeria
Pseudomonas
Staphylococcus
Morphology
Correlation
Palynology
Anatomy
Adaptation
Funding
None.
References
  1. Parekh J, Chanda S. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol. 2007;31:53-58.

 

  1. Anwar F, Qayyum HMA, Hussain AI, Iqbal S. Antioxidant activity of 100% and 80% methanol extracts from barley seeds (Hordeum vulgare L.): Stabilization of sunflower oil. Grasas Aceites. 2010;61(3):237-243. doi: 10.3989/gya.087409

 

  1. Ismail NA, Matawali A, Kanak FA, et al. Antimicrobial activities and phytochemical properties of Blumea balsamifera against pathogenic microorganisms. J Med Life. 2022;15(8):951-954. doi: 10.25122/jml-2021-0296

 

  1. Kozłowska M, Scibisz I, Przybył JL, et al. Antioxidant and antibacterial activity of extracts from selected plant material. Appl Sci. 2022;12(19):9871. doi: 10.3390/app12199871

 

  1. Hussain A, Hayat MQ, Sahreen S, Bokhari SAI. Unveiling the foliar epidermal anatomical characteristics of genus Artemisia (Asteraceae) from Northeast (Gilgit-Baltistan), Pakistan. Int J Agric Biol. 2019;21(3):630-638. doi: 10.17957/IJAB/15.0938

 

  1. Janaćković P, Gavrilović M, Rančić D, Dajić-Stevanović Z, Giweli AA, Marin PD. Comparative anatomical investigation of five Artemisia L. (Anthemideae, Asteraceae) species in view of taxonomy. Braz J Bot. 2019;42:135-147. doi: 10.1007/s40415-019-00521-6

 

  1. Aati HY, Perveen S, Orfali R, et al. Chemical composition and antimicrobial activity of the essential oils of Artemisia absinthium, Artemisia scoparia, and Artemisia sieberi grown in Saudi Arabia. Arab J Chem. 2020;13(11):8209-8217. doi: 10.1016/j.arabjc.2020.09.055

 

  1. Hussain A. The genus Artemisia (Asteraceae): A review on its ethnomedicinal prominence and taxonomy with emphasis on foliar anatomy, morphology, and molecular phylogeny. Proc Pak Acad Sci B Life Environ Sci. 2020;57(1):1-28.

 

  1. El-Amier YA, Al Borki AES, Elagami SA. Potential of wild plant Artemisia judaica L. as sustainable source of antioxidant and antimicrobial compounds. J Exp Sci. 2019;10:4-8. doi: 10.25081/jes.2019.v10.5425

 

  1. Bordean ME, Ungur RA, Toc DA, et al. Antibacterial and phytochemical screening of Artemisia species. Antioxidants (Basel). 2023;12:596. doi: 10.3390/antiox12030596

 

  1. Abd EL-Wahab RH, Zayed AEM, Moustafa AER, Klopatek JM, Helmy MA. Landforms, vegetation, and soil quality in South Sinai, Egypt. CATRINA. 2006;1(2):127-138.

 

  1. Atta E, Al faifi T, El-Shabasy A. Chemotaxonomic and morphological classification of six Indigofera species in Jazan region, KSA. J Saudi Chem Soc. 2022;26(3):101476. doi: 10.1016/j.jscs.2022.101476

 

  1. Chaudhary S, Ali Z, Tehseen M, et al. Efficient in planta production of amidated antimicrobial peptides that are active against drug-resistant ESKAPE pathogens. Nat Commun. 2023;14:1464. doi: 10.1038/s41467-023-37003-z

 

  1. Murray AK, Moore TL, Manning JB, Griffiths CE, Herrick AL. Noninvasive measurement of skin autofluorescence is increased in patients with systemic sclerosis: An indicator of increased advanced glycation endproducts? J Rheumatol. 2012;39(8):1654-1658. doi: 10.3899/jrheum.111359

 

  1. Ripolles-Avila C, Ramos-Rubio M, Hascoёt AS, Castillo M, Rodríguez-Jerez JJ. New approach for the removal of mature biofilms formed by wild strains of Listeria monocytogenes isolated from food contact surfaces in an Iberian pig processing plant. Int J Food Microbiol. 2020;323:108595.doi: 10.1016/j.ijfoodmicro.2020.108595

 

  1. Ochoa SA, López-Montiel F, Escalona G, et al. Pathogenic characteristics of Pseudomonas aeruginosa strains resistant to carbapenems associated with biofilm formation. Bol Med Hosp Infant Mex. 2013;70(2):133-144.

 

  1. Fábrega A, Vila J. Salmonella enterica serovar typhimurium skills to succeed in the host: Virulence and regulation. Clin Microbiol Rev. 2013;26(2):308-341. doi: 10.1128/cmr.00066-12

 

  1. Harshey RM. Bacterial motility on a surface: Many ways to a common goal. Annu Rev Microbiol. 2013;57:249-273. doi: 10.1146/annurev.micro.57.030502.091014

 

  1. Harris LG, Foster SJ, Richards RG. An introduction to Staphylococcus aureus, and techniques for identifying and quantifying S. aureus adhesins in relation to adhesion to biomaterials: Review. Eur Cells Mater. 2002;4:39-60. doi: 10.22203/eCM.v004a04

 

  1. Treangen TJ, Maybank RA, Enke S, et al. Complete genome sequence of the quality control strain Staphylococcus aureus subsp. aureus ATCC 25923. Genome Announc. 2014;2(6):10-14. doi: 10.1128/genomea.01110-14

 

  1. Bangchang KN, Cheoymang A, Muhamad N, Kulma I. Bioassay for total serum bioactivity of Atractylodes lancea. J Adv Pharm Technol Res. 2023;14(1):51-55. doi: 10.4103/japtr.japtr_431_22

 

  1. Kostić M, Smiljković M, Petrović J, et al. Chemical, nutritive composition and a wide range of bioactive properties of honey mushroom Armillaria mellea (Vahl: Fr.) Kummer. Food Funct. 2017;8(9):3239-3249. doi: 10.1039/c7fo00887b

 

  1. Joshi RK. In vitro antimicrobial and antioxidant activities of the essential oil of Craniotome furcata. J Appl Nat Sci. 2010;2: 57-62. doi: 10.31018/jans.v2i1.95

 

  1. Hayat MQ, Ashraf M, Jabeen S, Shaheen N, Yasmin G, Khan MA. Taxonomic implications of foliar epidermal characteristics with special reference to stomatal variations in the genus Artemisia (Asteraceae). Int J Agric Biol. 2010;12(2):3-6.

 

  1. Shmygareva AA, Bondarenko AI, Kurkin VА, San’Kov AN, Dorohina OA, Kochukova AA. Comparative morphological and anatomical studies of Artemisia absinthium and Artemisia austriaca. Der Pharma Chem. 2019;11(2):14-19.

 

  1. Rubina A, Qaiser M. Cypsela morphology and its taxonomic significance of the genus Artemisia L. (Anthemideae- Asteraceae) from Pakistan. Pak J Bot. 2008;40(5):1827-1837.

 

  1. Hayat MQ, Ashraf M, Khan MA, Yasmin G, Shaheen N, Jabeen S. Palynological study of the genus Artemisia (Asteraceae) and its systematic implications. Pak J Bot. 2010;42(2):751-763.

 

  1. Hussain A, Potter D, Hayat MQ, Sahreen S, Bokhari SAI. Pollen morphology and its systematic implication on some species of Artemisia L. from Gilgit-Baltistan Pakistan. Bangladesh J Plant Taxon. 2019;26(2):157-168 doi: 10.3329/bjpt.v26i2.44576

 

  1. Shaban, N. Analysis of the correlation and regression coefficients of the interaction between yield and some parameters of snap beans plants. Trakia J Sci. 2005;3(6):27-31.

 

  1. Dutilleul P. Modifying the t test for assessing the correlation between two spatial processes. Biometrics. 1993;49:305-314.

 

  1. Al Faifi T, El-Shabasy A. Effect of heavy metals in the cement dust pollution on morphological and anatomical characteristics of Cenchrus ciliaris L. Saudi J Biol Sci. 2021;28(1):1069-1079. doi: 10.1016/j.sjbs.2020.11.015

 

  1. Maindonald JH. Statistical design, analysis, and presentation issues. N Z J Agric Res. 1992;35(2):121-141. doi: 10.1080/00288233.1992.10417710

 

  1. Miller J, Franklin J. Modeling the distribution of four vegetation alliances using generalized linear models and classification trees with spatial dependence. Ecol Model. 2002;157(2-3): 227-247. doi: 10.1016/S0304-3800(02)00196-5

 

  1. Kebede T, Gadisa E, Tufa A. Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: A possible alternative in the treatment of multidrug-resistant microbes. PLoS One. 2021;16:e0249253. doi: 10.1371/journal.pone.0249253

 

  1. Sadek P. Solvent Miscibility and Viscosity Chart adapted from The HPLC Solvent Guide. New York: Wiley-Interscience; 2002. p. 12.

 

  1. Hrytsyk RA, Kutsyk RV, Yurchyshyn OI, Struk OA, Kireev IV, Grytsyk AR. The investigation of antimicrobial and antifungal activity of some Artemisia L. species. Pharmacia. 2021;68(1):93-100. doi: 10.3897/pharmacia.68.47521

 

  1. Riahi L, Chograni H, Elferchichi M, Zaouali Y, Zoghlami N, Mliki A. Variations in Tunisian wormwood essential oil profiles and phenolic contents between leaves and flowers and their effects on antioxidant activities. Ind Crops Prod. 2013;46:290-296. doi: 10.1016/j.indcrop.2013.01.036

 

  1. Msaada K., Salem N., Bachrouch O., Bousselm S., Tammar S., Alfaify A. et al. Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics. J. Chem. 2015; 1: 804658. doi: 10.1155/2015/804658

 

  1. Lommen WJM, Schenk E, Bouwmeester HJ, Verstappen FWA. Trichome dynamics and artemisinin accumulation during development and senescence of Artemisia annua leaves. Planta Med. 2006;72:336-345. doi: 10.1055/s-2005-916202

 

  1. Nguyen HT, Radácsi P, Gosztola B, Németh ÉZ. Effects of temperature and light intensity on morphological and phytochemical characters and antioxidant potential of wormwood (Artemisia absinthium L.). Biochem Syst Ecol. 2018;79:1-7. doi: 10.1016/j.bse.2018.03.005

 

  1. Srilakshmi P, Naidu KC. A study on foliar epidermal features in Artemisia, Chrysanthemum and Cosmos of the family Asteraceae. IJAPBC. 2014;3(1):2-3.

 

  1. Sharifi-Rad J, Herrera-Bravo J, Semwal P, et al. Artemisia spp.: An update on its chemical composition, pharmacological and toxicological profiles. Oxid Med Cell Long. 2022;2022:5628601. doi: 10.1155/2022/5628601

 

  1. Lu LL, Jiao BH, Qin F, et al. Artemisia pollen dataset for exploring the potential ecological indicators in deep time. Earth Syst Sci Data. 2022;14:3961-3995. doi: 10.5194/essd-14-3961-2022

 

  1. Moslemi HR, Hoseinzadeh H, Badouei MA, Kafshdouzan K, Fard RM. Antimicrobial activity of Artemisia absinthium against surgical wounds infected by Staphylococcus aureus in a rat model. Indian J Microbiol. 2012;52:601-604. doi: 10.1007/s12088-012-0283-x

 

  1. Erdtman G. Pollen Morphology and Plant Taxonomy- Angiosperms. Waltham: Chronica Botanica; 1952. p. 222.

 

  1. Erdtman G. Suggestions for the classification of fossil and recent pollen grains and spores. Svensk Bot Tidskr. 1947;41:104-114.

 

  1. Straka H. Palynology of Madagascar and Mascarenica. Preface I. - Introduction II. Pollen Spores. 1964;6:239-283.

 

  1. Proctor M, Yeo P. The Pollination of Flowers. Collins, London, England: Cornell University; 1973. p. 418.

 

  1. Erdtman G. An Introduction to Pollen Analysis. Waltham, Massachusetts: Chronica Botanica Company; 1943. p. 239.

 

  1. Fritzsche J. About the pollen. Mém. Sav. Étrang. Acad Sci Pétersbourg. 1837;3:649-672.

 

  1. Iversen J, Troels-Smith J. Pollen morphology definitions and types. Danm Geol Unders Ser. 1950;3(8):1-54.

 

  1. Wodehouse RP. Pollen Grains: Their Structure, Identification and Significance in Science and Medicine. New York: McGraw- Hill; 1935. p. 574.

 

  1. Knoll F. About pollenkitt and pollination type. Z Botanik. 1930;23:610-675.

 

  1. Kuprianova LA. Pollen morphology of the monocotyledons. (Basic data on the phylogeny of classes). Flora and systematics of the vascular plants. Tr Botan Inst Akad. 1948;7:163-172.

 

  1. Balme BE. Miospores from Late devonian (early frasnian) strata, Carnarvon Basin, Western Australia. Palaeonthographica Abteilung B. 1988;209:109-166.
Conflict of interest
The authors declared no potential competing interests.
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Journal of Biological Methods, Electronic ISSN: 2326-9901 Print ISSN: TBA, Published by POL Scientific
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