Background: Neurological diseases are pathologies that affect the central nervous system, such as Alzheimer’s disease and other forms of dementia. A correlation between cognitive decline and energy metabolism has been identified, and the potential to treat mental deterioration through the integration of natural mixtures into therapeutic strategies has gained increasing interest. Objective: Given the close relationship between hypometabolism and cognitive impairment, this study investigated the metabolic effects of a mixture containing medium-chain triglycerides, omega-3 fatty acids, and choline bitartrate in a human neuronal cell model, with relevance to cognitive decline. This was achieved by comparing the synergistic effects of the combined ingredients with the effects of the individual ingredients on energy metabolism (ATP) in a human neuronal model. Methods: Starting from the maximum non-toxic concentration common to both the mixture and individual components (1.563 mg/mL), the selected cell model (SH-SY5Y) was incubated for 24 h with decreasing concentrations of the mixture to evaluate ATP levels and to identify the optimal concentration (0.391 mg/mL). The cell viability results of the mixture were compared with the dose–response curves generated from cells treated with the individual components at equivalent concentrations to those present in the mixture. Results: A statistically significant increase (p<0.05) was observed in ATP content following treatment with the mixture compared to the individual active ingredients. Conclusion: The pre-clinical results demonstrate that a mixture containing medium-chain triglycerides, omega-3 fatty acids, and choline bitartrate exerts a synergistic effect on cellular energy metabolism, enhancing ATP production in a neuronal model. This formulation supports energy metabolism and may be beneficial for patients suffering from neurodegenerative diseases.