POL Scientific / JBM / Volume 8 / Issue 1 / DOI: 10.14440/jbm.2021.348
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Visualization of subdiffusive sites in a live single cell

Zeno Földes-Papp1 Gerd Baumann2 Long-Cheng Li3
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1 Head of the Department of Geriatrics, Asklepios Clinic Lindau, 88131 Lindau (at Lake Constance), Bavaria, Germany
2 Head of the Mathematics Department, Faculty of Basic Sciences, German University in Cairo (GUC), 11835 New Cairo City, Egypt
3 Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong 226001, China
Published: 30 January 2021
© 2021 by the author. Licensee POL Scientific, USA. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

We measured anomalous diffusion in human prostate cancer cells which were transfected with the Alexa633 fluorescent RNA probe and co-transfected with enhanced green fluorescent protein-labeled argonaute2 protein by laser scanning microscopy. The image analysis arose from diffusion based on a “two-level system”. A trap was an interaction site where the diffusive motion was slowed down. Anomalous subdiffusive spreading occurred at cellular traps. The cellular traps were not immobile. We showed how the novel analysis method of imaging data resulted in new information about the number of traps in the crowded and heterogeneous environment of a single human prostate cancer cell. The imaging data were consistent with and explained by our modern ideas of anomalous diffusion of mixed origins in live cells. Our original research presented in this study is significant as we obtained a complex diffusion mechanism in live single cells.

Keywords
anomalous diffusion
complex diffusion mechanism
imaging
live cell
number of traps
References

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Journal of Biological Methods, Electronic ISSN: 2326-9901 Print ISSN: TBA, Published by POL Scientific