POL Scientific / JBM / Volume 4 / Issue 4 / DOI: 10.14440/jbm.2017.203
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Easy and cost-effective stable positioning of suspension cells during live-cell imaging

Daniel Ivanusic1 Kazimierz Madela1 Joachim Denner1
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1 Robert Koch Institute, Berlin, Germany
JBM 2017 , 4(4), 1;
Published: 17 October 2017
© 2017 by the author. Licensee POL Scientific, USA. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

Dynamic processes of cells can be best monitored when living cells are analyzed by imaging. While it is easy to observe adherent living cells it has been extremely challenging to analyze suspension cells. This cell type floats freely in the culture dish, and it is only a question of time when the focus or the observation field is lost. In order to keep the cells in focus, an easy and inexpensive method allowing the observation of living suspension cells during confocal laser scanning microscope imaging was developed.

Keywords
live-cell imaging
suspension culture
confocal laser scanning microscope
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Journal of Biological Methods, Electronic ISSN: 2326-9901 Print ISSN: TAB, Published by POL Scientific