POL Scientific / JBM / Volume 4 / Issue 3 / DOI: 10.14440/jbm.2017.180
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Measurement of intracellular calcium of submandibular glands using a high throughput plate reader

Abeer Kamal Shaalan1 Guy Carpenter1 Gordon Proctor1
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1 Mucosal and Salivary Biology Division, Dental Institute, King’s College London, Guy’s Hospital, Floor 17, Tower Wing, London SE1 9 RT, UK
Published: 17 July 2017
© 2017 by the author. Licensee POL Scientific, USA. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

Calcium ions (Ca2+) impact nearly every aspect of cellular life and intracellular calcium [Ca2+]i is a critical factor in the regulation of a plethora of physiological functions, including: muscle contraction, saliva secretion, metabolism, gene expression, cell survival and death. By measuring the changes of [Ca2+]i levels, critical physiologic functions can be characterized and aberrant pathologic conditions or drug responses can be efficiently monitored. We developed a protocol for assessment of Ca2+ signaling in the acinar units of submandibular glands isolated from C57BL/6 mice, using benchtop, multi-mode, high throughput plate reader (FlexStation 3). This method represents a powerful tool for unlimited in vitro studies to monitor changes in receptor-mediated Ca2+ responses while retaining functional and morphological features of a native setting.

Keywords
calcium
carbachol
in vitro
FlexStation
ionomycin
salivary glands
signaling
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Journal of Biological Methods, Electronic ISSN: 2326-9901 Print ISSN: TBA, Published by POL Scientific