Development of a method for DNA extraction from oil palm leaves and the effects of pH and ionic strength on nucleic acid quantification
Oil palm (Elaeis guineensis Jacq.) has benefited little from molecular marker technologies although the long generation time and the unpredictable variations due to allogamy make it a high priority for improving breeding efficiency. Progress would require an efficient, low cost and high-throughput deoxyribonucleic acid (DNA) extraction method adapted for oil palm. A method for genomic DNA extraction from oil palm leaves that is simple and cost-effective was developed without the use of liquid nitrogen. A260/280 ratios between 1.987 and 2.078 were obtained. Furthermore, we could demonstrate that nucleic acid concentration and yield were inversely proportional to the pH and ionic strength of the solvent. The measured values varied significantly between 572 ng/µl and 496 ng/µl. DNA extracted with this method is stable and can be reproducibly amplified by PCR.
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