POL Scientific / JBM / Volume 3 / Issue 2 / DOI: 10.14440/jbm.2016.112
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Assessing bioenergetic functions from isolated mitochondria in Drosophila melanogaster

Wen C. Aw1 Rijan Bajracharya1 Samuel G. Towarnicki1 J. William O. Ballard1
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1 School of Biotechnology and Biomolecular Science, University of New South Wales, Sydney 2052, Australia
Published: 8 June 2016
© 2016 by the author. Licensee POL Scientific, USA. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

Mitochondria are involved in generating more than 90 percent of cellular energy and are responsible for many cellular processes such as metabolism, cell signalling, apoptosis and ageing. Currently, there are a number of different experimental approaches employed to measure mitochondrial health and function. Here, we demonstrate a novel approach that quantifies substrate induced mitochondrial respiration from Drosophila. This protocol is optimized for mitochondria isolated from third instar larvae, and can also be used for mitochondria isolated from adult thoraces. This procedure outlines how to perform high throughput and high resolution mitochondria specific measurements for state II, state III, state IVO respiration and residual oxygen consumption.

Keywords
Drosophila
mitochondrial isolation
bioenergetics
seahorse XF
complex I
larvae
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Journal of Biological Methods, Electronic ISSN: 2326-9901 Print ISSN: TBA, Published by POL Scientific